The Protein Science Translation Core provides direct support to investigators of the COBRE in Lipidomics and Pathology program, as well as other investigators at the Medical University of South Carolina (MUSC), in the production of recombinant proteins via prokaryotic and eukaryotic expression systems and in the structural and functional characterizations of proteins. The Core also supports translational research by producing active proteins that are needed for high-throughput screening assays and for determining the mechanisms and efficacy of various synthetic compounds. In addition to service, an important commitment of the Core is to mentor and assist investigators in solving technical problems concerning protein expression, purification, and characterization.
The Core also provides a wide range of protein-related specialty services to suit the needs of the investigator. This includes the determination of the physical state of the protein by circular dichroism (CD), dynamic light scattering (DLS) and protein crystallization. In addition, the Core will assist to investigate protein-protein interactions by immunoprecipitation and Western blotting. Furthermore we will assist to investigate protein-lipid interactions by fat western and HPLC-based enzyme assays.
Specialty Protein Services Provided