Fat Western is used to screen for protein-lipid interactions. Using a dot-blot of a selected set of lipids one can determine the lipid-binding specificity of a particular target protein. In addition, it can be used to identify the lipid-binding domains within a target protein. Moreover, the assay permits an estimation of the affinity constant. Technically, fat westerns are based on immunoblotting techniques in which lipid-protein binding precedes the antibody detection steps.
The Core provides assistance in experimental design, and training to conduct assays, and data interpretation.
In the next phase, we will develop a Surface Plasmon Resonance (SPR)-based protein lipid assay.
Specialty Protein Services Provided