Small-scale Protein Preparation Small-scale Protein Preparation

A series of small-scale induction experiments will be conducted to optimize the type vector, the E.coli strain, expression temperature, media and other parameters.

 

Vector Construct
List of selected expression vectors used by the Core
pET series  His6-tag
pT7HTb His6-tag/TEV cleavage
pGEX series GST fusion
pMAL-C2KV MBP fusion/TEV cleavage
pTYB series Chitin bhinding domain fusion

 

E.coli Strain
List of E. coli strains used by the Core
BL21 (DE3) pLysS
BL21 (DE3) codon plus
DH 5 alpha
LMG 194
Origami 2
Rosetta
Top 10

 

An SDS-PAGE of a small-scale induction experiment performed by the Core. Presented with kind permission of Dr. C. Luberto, Biochemistry and Molecular Biology, MUSC)

An SDS-PAGE of a small-scale induction experiment performed by the Core.
Presented with permission from Dr. C. Luberto, Biochemistry and Molecular Biology, MUSC

 

Contacts Contacts

Director: Yi-Te Hsu, Ph.D. Associate Professor
hsuy@musc.edu
Tel: 843-792-0849
Co-Director: Erika E. B├╝llesbach, Ph.D. Associate Professor
bullesee@musc.edu Tel: 843-792-9926
Research Specialist II: Mr. Dzmitry Fedarovich
fedarov@musc.edu Tel: 843-792-6529