Large-scale production of recombinant proteins is necessary to produce the quantities of protein for detailed analyses, i.e., probing for protein-ligand interactions, producing antibodies, characterizing enzymatic properties, and facilitating structural studies by X-ray crystallography or NMR spectroscopy. This requires expressing the protein at the optimized conditions, which should yield tens of milligrams of target protein per liter of culture. The Core is equipped with an 11-L fermenter to provide the culture volume and with chromatography equipment to purify such amounts of proteins. We have also conducted large-scale refolding of proteins from inclusion bodies.
Below is an example of a protein produced by large-scale induction and its purity after affinity chromatography and concentration. Presented with kind permission from Dr. E. Spicer, Biochemistry and Molecular Biology, MUSC.
|CD Spectrum indicating the protein is structured|
|SDS-PAGE at different stages during production and purification|
1. unpurified sample
Specialty Protein Services Provided