The Core provides customers with fast and high precision electrophoresis techniques, which allow verifying homogeneity, molecular weight, and isoelectric point of the protein using a programmable PhasteSystem, GE Healthcare, electrophoresis instrument. The laboratory uses precast SDS- and native-polyacrylamide gels of various pore-size and gradients allowing the use of most electrophoresis buffers.
Proteins are visualized with Coomassie Brilliant Blue, silver staining or by a reverse protein stain. In addition, the laboratory provides full cycle preparation for drying, scanning, digital processing, analysis and documentation of Coomassie- and silver-stained gels.
The core is equipped with a programmable PhasteSystem (GE Healthcare)
1. Eluted protein
2. Concentrated up to ~12 mg/ml
3. ~18-20 mg/ml
4. ~9 mg/ml
Often it is useful and time efficient to check the molecular weight and the native state of a protein side by side. The example below shows polyacrylamide gel-electrophoreses of the same protein in SDS-containing buffer (left) and native buffer (right), indicating that a single analytical electrophoresis system is not sufficient to prove homogeneity.
Protein after affinity chromatography analyzed by SDS-PAGE (left) and native gel (right)
Specialty Protein Services Provided