For X-ray crystallographic studies, the Core can assist investigators with crystal screening trials and process monitoring. Prior to the crystallization trial, the protein sample will be prepared to meet certain conditions such as concentration, buffer, purity, aggregation state etc. For crystallization, standard sitting or hanging drop vapor diffusion method at different temperatures will be carried out. Crystallization will be monitored by light microscopy and protein crystals will be subsequently identified by X-ray diffraction.
Example of a protein produced by large-scale induction and its subsequent crystallization. Left: purity of Pim1 kinase used in crystallization studies. Right: crystals. (With kind permission of Dr. Zemskova, Dr. Kraft's lab, Holling's Cancer Center, MUSC)
Specialty Protein Services Provided