HPLC techniques allow the separation of chemically defined compounds from complex mixtures. The substance can be quickly identified by their molecular mass and fragmentation pattern through mass spectrometry. By using appropriate standards individual compounds can be quantified. HPLC lends itself to conduct reaction kinetics, especially enzyme kinetics, by quantifying the time-dependent decline of substrate and simultaneous increase of product(s).
The advantage of using the MS-detector is to perform enzyme kinetics with natural enzyme substrates. The instrument will be used for lipid or protein/peptide analyses and is located in room BSB 741.
The Core will provide assistance in experimental design, training on the usage of the equipment and software and data interpretation.
The Thermo Finnigan LCQ LC-MS is interfacedwith an Agilent Surveyor HPLC. The eluate can be directly analyzed by MS or samples can be infused into the LCQ without prior chromatography.
Specialty Protein Services Provided